Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Reference annotation for mapping (human)?

    Question:
    Which annotation is best for reference-based/guided mapping and assembly using Tophat/Cufflinks/other tools?

    UCSC Comprehensive Gencode11 track, downloaded as a .gtf, has 2072303 records.

    ENSEMBL66 [Homo_sapiens.GRCh37.66.gtf] has 2181086 records.

    gencode11 (on which ENSEMBL is based(???)) has 2617472 records, of which:
    1947538 gencode.v11.annotation.level_1_2.gtf
    586834 gencode.v11.annotation.level_3.gtf
    74062 gencode.v11.polyAs.gtf
    9038 gencode.v11.2wayconspseudos.gtf

    Or, what are the pros/cons of using each of them? And what is the actual difference?

    Thanks in advance!

  • #2
    *bumping up my own post, since this is still relevant*
    (Thanks in advance!)

    Comment

    Latest Articles

    Collapse

    • noor121
      Reply to Latest Developments in Precision Medicine
      by noor121
      Qadri offers efficient online services designed for students and staff of University Targu Mures Medical Campus Hamburg. We streamline your academic and administrative processes for a hassle-free experience.

      VIsit us:
      https://qadri-international.com/univ...s-hamburg-umch...
      Today, 09:33 PM
    • seqadmin
      Non-Coding RNA Research and Technologies
      by seqadmin




      Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.

      Nobel Prize for MicroRNA Discovery
      This week,...
      Yesterday, 08:07 AM
    • seqadmin
      Recent Developments in Metagenomics
      by seqadmin





      Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...
      09-23-2024, 06:35 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 10-02-2024, 04:51 AM
    0 responses
    96 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 10-01-2024, 07:10 AM
    0 responses
    107 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-30-2024, 08:33 AM
    1 response
    109 views
    0 likes
    Last Post EmiTom
    by EmiTom
     
    Started by seqadmin, 09-26-2024, 12:57 PM
    0 responses
    20 views
    0 likes
    Last Post seqadmin  
    Working...
    X