Hi All,
I have a range of chromosome coordinates i.e.
From this range, I have to pull out a subsequence of 200-400 nucleotides. The subsequence shouldn't appear in another part of the human genome. This should be an unique sequence. (The best case, the subsequence would be an exon of HERC6, but not necessary)
Do you have any idae how to automate this process? Which tools/services should I use?
I know I can do this in such way:
I have a range of chromosome coordinates i.e.
Code:
HERC6 4:89,280,000-89,350,000
Do you have any idae how to automate this process? Which tools/services should I use?
I know I can do this in such way:
HTML Code:
Ensembl -> type in the search box "4:89,280,000-89,350,000" -> export data -> cut a subsequence -> paste to the blast. But this way is not satisfactory.
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