Hi guys
Following the indications suggested in other threads I am constructing an exome pipeline for SOLID data based on the combination of BWA with picard and GATK and I wonder about if in the GATK step of variant quality score recalibration could be a good (or bad) idea if I get exome bam files at 1000genomes aligned with BFAST and merge them with my exome bam file aligned with BWA to improve the coverage of the latter in the subsequent score recalibration.
Thanks in advance
Carlos
Following the indications suggested in other threads I am constructing an exome pipeline for SOLID data based on the combination of BWA with picard and GATK and I wonder about if in the GATK step of variant quality score recalibration could be a good (or bad) idea if I get exome bam files at 1000genomes aligned with BFAST and merge them with my exome bam file aligned with BWA to improve the coverage of the latter in the subsequent score recalibration.
Thanks in advance
Carlos