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How is FASTQC determining encoding in basic summary? Their documentation doesn't seem to cover anything in regard?
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Well, you probably should write to the authors if you want to know. As a guess I suspect that it is looking at all of the quality values and then determining the lowest and highest value it sees. From there it would be quite easy to figure out the encoding used.
In other words, given only one sequence it is hard to tell what the encoding is. Given 1,000,000 sequences it would be trivial.
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
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