Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Qsub bwa pipeline...

    This may seem like a relatively easy question, but here goes:

    I am trying to create a relatively simple shell script that sits in a parent folder, goes through all subfolders, grabs all files with the extension .fq, and aligns them using BWA aln. The caveat is that I need to use qsub to do this or I will make the admins very unhappy

    My initial script was something along the lines of the following:

    result=(`find . -name "*.fq" -type f`)

    for i1 in ${result[@]}
    do

    qsub -q queue_name -l nodes=1pn=8 -V test.sh

    done

    It should be noted that the first portion simply gets the filenames (and paths) and the second calls test.sh, which is shown below (note that the first two variable paths are truncated for forum purposes):

    REFERENCE=/refpath
    BWA_HOME=/bwapath

    result=(`find . -name "*.fq" -type f`)

    for i1 in ${result[@]}
    do

    $BWA_HOME/bwa aln -t 8 $REFERENCE $i1 > $i1".sai"

    done

    I am simply trying to get BWA to run using the $1i filename and $i1.sai as the output name. The reason I ran the for loop here and in the initial script is because there seems to be a problem holding on to $i1. If I run the bottom script as a standalone script, BWA begins running on the first file. The key is I'm not certain how to get it to run qsub X times (where X is the number of .fq files), each time is with a different file/output. Any help would be greatly appreciated. Thanks!

  • #2
    Anyone?

    Comment


    • #3
      What if you change your test.sh to just take one .fq file at a time and you pass the file name to test.sh in the for-loop of your initial script?

      Comment


      • #4
        If you are interested check out our pipeline (www.keatslab.org). Its a qsub based program. We build two flavours. One that reads all the fastq pairs in a folder and aligns then calls variants. The other leverages an input file that allows for proper RG tag assignment that is required for GATK variant calling. Nice thing is it will merge multiple lanes of the same library together for you.

        Comment


        • #5
          You'll probably find it more convenient to use a single job array than submitting many jobs in a loop.
          In your launch script do a single qsub:
          qsub -t 0-$[${#result[@]}-1] test.sh
          In test.sh the environment variable PBS_ARRAYID tells you which job is running:
          i1=${result[$PBS_ARRAYID]}

          Comment


          • #6
            Got it working using:

            sh "/filepath/test.sh $i1" | qsub -q queue_name -l nodes=1pn=8

            Not positive why I had to go about it in such a roundabout way.

            Comment

            Latest Articles

            Collapse

            • seqadmin
              Recent Advances in Sequencing Analysis Tools
              by seqadmin


              The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
              Yesterday, 07:48 AM
            • seqadmin
              Essential Discoveries and Tools in Epitranscriptomics
              by seqadmin




              The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
              04-22-2024, 07:01 AM

            ad_right_rmr

            Collapse

            News

            Collapse

            Topics Statistics Last Post
            Started by seqadmin, Today, 06:57 AM
            0 responses
            9 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, Yesterday, 07:17 AM
            0 responses
            14 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 05-02-2024, 08:06 AM
            0 responses
            19 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-30-2024, 12:17 PM
            0 responses
            23 views
            0 likes
            Last Post seqadmin  
            Working...
            X