I recently sequenced some bisulfite converted DNA and some unconverted DNA in the same lane of a MiSeq. In the bisulfite converted DNA I had spiked in a small amount of unmethylated lambda DNA as a control. In the unconverted DNA I did not spike in the lambda control. I mapped both the unconverted and converted DNA to the lambda genome with stringent mapping parameters using Bismark. Both samples gave me alignments. It appears that the unconverted sample mapped only to the unconverted lambda genome where as the converted samples mapped to converted lambda genomes. However, how can I confidently determine my conversion efficiency if the results could be due to nonspecific mapping? Has anyone had this problem?
-
The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.Today, 06:37 PM -
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
| Topics | Statistics | Last Post | ||
|---|---|---|---|---|
|
0 responses
27 views
0 reactions
|
by SEQadmin2
11-12-2026, 07:59 AM
|
|||
|
0 responses
35 views
0 reactions
|
by SEQadmin2
11-05-2026, 09:25 AM
|
|||
|
2 responses
56 views
0 reactions
|
by kinpict.com
Today, 06:49 PM
|
|||
|
0 responses
175 views
0 reactions
|
by SEQadmin2
10-28-2026, 09:57 AM
|