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I recently sequenced some bisulfite converted DNA and some unconverted DNA in the same lane of a MiSeq. In the bisulfite converted DNA I had spiked in a small amount of unmethylated lambda DNA as a control. In the unconverted DNA I did not spike in the lambda control. I mapped both the unconverted and converted DNA to the lambda genome with stringent mapping parameters using Bismark. Both samples gave me alignments. It appears that the unconverted sample mapped only to the unconverted lambda genome where as the converted samples mapped to converted lambda genomes. However, how can I confidently determine my conversion efficiency if the results could be due to nonspecific mapping? Has anyone had this problem?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
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