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Hello
We analyse Solid 5500 RNA seq reads. I used tophat2 for the alignment. We saw some odd results relative to the mapping quality. All quality are 255... So that none of them are going to be filtered relative to their quality. Have you ever seen this issue with TopHat2 please? How did you fix it?
Thanks for your time
Virginie
We analyse Solid 5500 RNA seq reads. I used tophat2 for the alignment. We saw some odd results relative to the mapping quality. All quality are 255... So that none of them are going to be filtered relative to their quality. Have you ever seen this issue with TopHat2 please? How did you fix it?
Thanks for your time
Virginie
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