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  • vbernard
    Junior Member
    • Jul 2012
    • 6
    #1

    mapping quality tophat2 - always 255

    Hello
    We analyse Solid 5500 RNA seq reads. I used tophat2 for the alignment. We saw some odd results relative to the mapping quality. All quality are 255... So that none of them are going to be filtered relative to their quality. Have you ever seen this issue with TopHat2 please? How did you fix it?
    Thanks for your time
    Virginie
    Tags: None
  • vbernard
    Junior Member
    • Jul 2012
    • 6
    #2
    any help please?
    Thanks

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