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  • Does cufflink provide the variance of gene/transcript expression?

    Dear all

    I use the cufflink to calculate the expression of genes/transcripts-level. From the output files, I can obtain the FPKM values, but I don't find the variance of each gene/transcript.
    In our downstream analysis, I need use the variance of gene/transcript, so I have some problems:
    1) Does cufflink provide variance of gene/transcript expression?
    2) If provide, how to obtain the variance by setting cufflink's option?
    3) If not provide, how to calculate the variance by myself.


    Thank you !!
    Leo

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