Hi,
Can anyone tell me how to calculate a base's distance to 3' end respectively for the forward and reverse strand read. I want to konw if the following formula is correct?
1)for forward strand read: distance=the read's alignmnet end position-the base's position
2)for reverse strand read: distance=the base's position-the read's alignment start position
Thanks
Can anyone tell me how to calculate a base's distance to 3' end respectively for the forward and reverse strand read. I want to konw if the following formula is correct?
1)for forward strand read: distance=the read's alignmnet end position-the base's position
2)for reverse strand read: distance=the base's position-the read's alignment start position
Thanks
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