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Hi All,
I can't find any documentation regarding this. Please Help!
When I "Import From BLAST..." in MEGAN 4.64.2 on Linux 10.04 and specify a single blastx output file (e.g. 4 GB) in .txt format, and its corresponding single reads file (e.g. 50 MB) in .fna, and try to save it as temp.rma it successfully processes the reads file (progress bar goes to completion) then a new window/bar comes up mentioning the blast results and before the progress bar has a chance to move the whole program closes.
I'm left with temp.rma (2 MB) and temp.tmp0 (200 kb), and when I try to open the .rma from within MEGAN I get "Execute failed: RMA2 file corrupt? Expected: -1, got: -109"
I have 5 other similarly sized pairs of files, and this happens with all of them. The system has 8 cores and 24 GB RAM. I've used MEGAN before, importing some of these exact same files and performing analyses, without problems. I don't know what's changed since then, except that we've added more RAM, and a couple months of regular system updates.
Please Help!
I can't find any documentation regarding this. Please Help!
When I "Import From BLAST..." in MEGAN 4.64.2 on Linux 10.04 and specify a single blastx output file (e.g. 4 GB) in .txt format, and its corresponding single reads file (e.g. 50 MB) in .fna, and try to save it as temp.rma it successfully processes the reads file (progress bar goes to completion) then a new window/bar comes up mentioning the blast results and before the progress bar has a chance to move the whole program closes.
I'm left with temp.rma (2 MB) and temp.tmp0 (200 kb), and when I try to open the .rma from within MEGAN I get "Execute failed: RMA2 file corrupt? Expected: -1, got: -109"
I have 5 other similarly sized pairs of files, and this happens with all of them. The system has 8 cores and 24 GB RAM. I've used MEGAN before, importing some of these exact same files and performing analyses, without problems. I don't know what's changed since then, except that we've added more RAM, and a couple months of regular system updates.
Please Help!