Hi everyone,
I have called SNPs using SAMtools and now wish to use vcftools to filter my SNPs. I need to keep SNPs with at least 100 bp flanking both sides for primer design (i.e. no other SNPs present in 100 bp flanking region). Do you have any idea of how to get this work?
Any suggestion would be appreciated.
T.
I have called SNPs using SAMtools and now wish to use vcftools to filter my SNPs. I need to keep SNPs with at least 100 bp flanking both sides for primer design (i.e. no other SNPs present in 100 bp flanking region). Do you have any idea of how to get this work?
Any suggestion would be appreciated.
T.