Tweet
hi..
I have 454 Amplicon sequencing data for a tetraploid potato crop. we have sequenced few genes of interest from several different genotypes. My aim is to seperate genotypes and sub genomes here. I am thinking of using haplotype analysis to seperate the genotypes as well as sub genomes here.
As i am new to this, can someone point me to some tools / softwares which can take the input i have and give haplotypes as output? All i have is gene sequences, and mapped bam file and raw reads. I dont have any marker data or genotype data with me.. i know they are sampled form 10 genotype of one type and other 5 are of other type..
I have 454 Amplicon sequencing data for a tetraploid potato crop. we have sequenced few genes of interest from several different genotypes. My aim is to seperate genotypes and sub genomes here. I am thinking of using haplotype analysis to seperate the genotypes as well as sub genomes here.
As i am new to this, can someone point me to some tools / softwares which can take the input i have and give haplotypes as output? All i have is gene sequences, and mapped bam file and raw reads. I dont have any marker data or genotype data with me.. i know they are sampled form 10 genotype of one type and other 5 are of other type..