Hi all, when I run GATK VariantRecalibrator using the following commands: run_gatk.sh
-T VariantRecalibrator
-R GRCh37/human_g1k_v37.fasta -input raw4.snp.vcf
-resource:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap_3.3.b37.sites.vcf
-resource
mni,known=false,training=true,truth=false,prior=12.0 1000G_omni2.5.b37.sites.vcf
-resource:dbsnp,known=true,training=false,truth=false,prior=6.0 dbsnp_134.b37.vcf
-an QD -an HaplotypeScore -an MQRankSum -an ReadPosRankSum -an FS -an MQ -an InbreedingCoeff
-mode SNP
-recalFile raw4.snp.vcf.recal
-tranchesFile raw4.snp.vcf.tranches -rscriptFile raw4.snp.vcf.plots.R"
error message as follows:
ERROR ------------------------------------------------------------------------------------------
ERROR A USER ERROR has occurred (version 2.0-39-gd091f72):
ERROR The invalid arguments or inputs must be corrected before the GATK can proceed
ERROR Please do not post this error to the GATK forum
ERROR
ERROR See the documentation (rerun with -h) for this tool to view allowable command-line arguments.
ERROR Visit our website and forum for extensive documentation and answers to
ERROR commonly asked questions http://www.broadinstitute.org/gatk
ERROR
ERROR MESSAGE: Bad input: Values for InbreedingCoeff annotation not detected for ANY training variant in the input callset. VariantAnnotator may be used to add these annotations. See http://www.broadinstitute.org/gsa/wi...riantAnnotator
Which kind of annotations should I add and how? I'm quite worry, thanks a lot!
-T VariantRecalibrator
-R GRCh37/human_g1k_v37.fasta -input raw4.snp.vcf
-resource:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap_3.3.b37.sites.vcf
-resource
![Embarrassment](https://www.seqanswers.com/core/images/smilies/redface.png)
-resource:dbsnp,known=true,training=false,truth=false,prior=6.0 dbsnp_134.b37.vcf
-an QD -an HaplotypeScore -an MQRankSum -an ReadPosRankSum -an FS -an MQ -an InbreedingCoeff
-mode SNP
-recalFile raw4.snp.vcf.recal
-tranchesFile raw4.snp.vcf.tranches -rscriptFile raw4.snp.vcf.plots.R"
error message as follows:
ERROR ------------------------------------------------------------------------------------------
ERROR A USER ERROR has occurred (version 2.0-39-gd091f72):
ERROR The invalid arguments or inputs must be corrected before the GATK can proceed
ERROR Please do not post this error to the GATK forum
ERROR
ERROR See the documentation (rerun with -h) for this tool to view allowable command-line arguments.
ERROR Visit our website and forum for extensive documentation and answers to
ERROR commonly asked questions http://www.broadinstitute.org/gatk
ERROR
ERROR MESSAGE: Bad input: Values for InbreedingCoeff annotation not detected for ANY training variant in the input callset. VariantAnnotator may be used to add these annotations. See http://www.broadinstitute.org/gsa/wi...riantAnnotator
Which kind of annotations should I add and how? I'm quite worry, thanks a lot!
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