Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • MissDona
    Member
    • Nov 2011
    • 17

    #1

    error_Values for InbreedingCoeff annotation not detected for ANY training variant in

    Hi all, when I run GATK VariantRecalibrator using the following commands: run_gatk.sh
    -T VariantRecalibrator
    -R GRCh37/human_g1k_v37.fasta -input raw4.snp.vcf
    -resource:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap_3.3.b37.sites.vcf
    -resourcemni,known=false,training=true,truth=false,prior=12.0 1000G_omni2.5.b37.sites.vcf
    -resource:dbsnp,known=true,training=false,truth=false,prior=6.0 dbsnp_134.b37.vcf
    -an QD -an HaplotypeScore -an MQRankSum -an ReadPosRankSum -an FS -an MQ -an InbreedingCoeff
    -mode SNP
    -recalFile raw4.snp.vcf.recal
    -tranchesFile raw4.snp.vcf.tranches -rscriptFile raw4.snp.vcf.plots.R"

    error message as follows:

    ERROR ------------------------------------------------------------------------------------------

    ERROR A USER ERROR has occurred (version 2.0-39-gd091f72):

    ERROR The invalid arguments or inputs must be corrected before the GATK can proceed

    ERROR Please do not post this error to the GATK forum

    ERROR

    ERROR See the documentation (rerun with -h) for this tool to view allowable command-line arguments.

    ERROR Visit our website and forum for extensive documentation and answers to

    ERROR commonly asked questions http://www.broadinstitute.org/gatk

    ERROR

    ERROR MESSAGE: Bad input: Values for InbreedingCoeff annotation not detected for ANY training variant in the input callset. VariantAnnotator may be used to add these annotations. See http://www.broadinstitute.org/gsa/wi...riantAnnotator

    Which kind of annotations should I add and how? I'm quite worry, thanks a lot!
  • MissDona
    Member
    • Nov 2011
    • 17

    #2
    I removed "-an InbreedingCoeff" and added --maxGaussians 4 , problem resolved
    don't know if that's good

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    19 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...