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  • Galaxy tool to filter low mapping quality reads?

    Does anybody know if there is a way to remove low quality reads on Galaxy? it could be easily done using command line based bamtools and all I have to do is specify the map quality. Is there an equivalent version on galaxy? Or is there a published workflow that does it for you? Thanks.

  • #2
    there is a tool called filter FASTQ (Filter FASTQ reads by quality score and length) under GENERIC FASTQ MANIPULATION

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    • #3
      Galaxy filter quality help

      Hello,
      trying to use galaxy to perform NGS analysis.
      I have created a history that contains my datasets. Now when I go down to pull the filterQC application I dont see my datasets in the "library to filter". How do I pull my datasets into the stream?
      Tutorial shows how to get data from UCSC and also if I have a work flow created then I can do it, but without a workflow , if I have to just use one tool to do the job how do I get my datasets to show up for an operation?
      Thanks for ur time
      geneart

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      • #4
        filterQC

        Before using Filter FastQ you must use the FASTQ Groomer.

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