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You can use certain spicy whom genome as reference. Those RNA-seq mapped back to the region other than protein coding RNA also can plus the rRNA and tRNA annotation to give higher output
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Hi,
I am trying to predicate LncRNA from aPlant species. I have sequenced total RNA and then proceed to do the de novo assembly to get the transcriptome. Any idea about how to predicate the LncRNA target without reference genome?
searching for the CPC software and phyloCSF but how can perform them from the basic I don't really know? Thank you so much!
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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07-08-2024, 03:19 PM -
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