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  • liweixie
    replied
    You can use certain spicy whom genome as reference. Those RNA-seq mapped back to the region other than protein coding RNA also can plus the rRNA and tRNA annotation to give higher output

    Leave a comment:


  • sali
    replied
    Hi,
    I am trying to predicate LncRNA from aPlant species. I have sequenced total RNA and then proceed to do the de novo assembly to get the transcriptome. Any idea about how to predicate the LncRNA target without reference genome?
    searching for the CPC software and phyloCSF but how can perform them from the basic I don't really know? Thank you so much!

    Leave a comment:


  • Nnogueira
    started a topic miRNA target package/program

    miRNA target package/program

    Hi,

    I was able to obtain a list of miRNAs from my RNA-seq data, but now I would like to analyze potential targets.

    Is there any software or bioconductor's package that allows me to identify potential targets?

    Thanks in advance!

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  • seqadmin
    Exploring the Dynamics of the Tumor Microenvironment
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    The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
    07-08-2024, 03:19 PM

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