Dear all,
I have anaylsed a RNAseq dataset with triplicates and 2 conditionsat the level of differential gene expression with HTSeq and this worked well (make biological sens and candidates confirmed by other technic). So I am confident that dataset is ok.
I now try to do the analyis at the level of transcipts with DExseq and cuffdiff.
I visualise the data from cuffdiff with cummeRbund and her is the grah for dispertion. It looks like that dispersion is higher for genes than isoforms. Could someone comment onto this?
I have anaylsed a RNAseq dataset with triplicates and 2 conditionsat the level of differential gene expression with HTSeq and this worked well (make biological sens and candidates confirmed by other technic). So I am confident that dataset is ok.
I now try to do the analyis at the level of transcipts with DExseq and cuffdiff.
I visualise the data from cuffdiff with cummeRbund and her is the grah for dispertion. It looks like that dispersion is higher for genes than isoforms. Could someone comment onto this?