Dear all
I’m quit new in the field of next generation sequencing.
We recently started a whole exome sequencing project based on two siblings both having the same cancer. In total 4 libraries (2x tumor, 2x healthy DNA) will be created. Sequencing: 2x100bp, Illumina.
Which are appropriate/required analyses which need to be done by our bioinformaticians in order to be able to identify unique or shared SNPs (possibly germline) in these siblings?
How can they be distinguished from recurrent SNPs? And how is it with mutations in the germline DNA which are not listed in SNP databases?
Thanks!
I’m quit new in the field of next generation sequencing.
We recently started a whole exome sequencing project based on two siblings both having the same cancer. In total 4 libraries (2x tumor, 2x healthy DNA) will be created. Sequencing: 2x100bp, Illumina.
Which are appropriate/required analyses which need to be done by our bioinformaticians in order to be able to identify unique or shared SNPs (possibly germline) in these siblings?
How can they be distinguished from recurrent SNPs? And how is it with mutations in the germline DNA which are not listed in SNP databases?
Thanks!
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