Hi All.
Sorry to bother you. Is there a way to distinguish duplicates caused by high sequencing depth from the PCR artefacts?
One more question. Is it necessary to remove the duplicates in the transcription factor ChIP-Seq? In our TF ChIP-Seqs, It is often to see a high duplication level. It is not the case in the controls. I guess the duplicate is caused by high sequencing depth not by the PCR artefacts.
Wish your help! Thanks very much!
Best, Mei
Sorry to bother you. Is there a way to distinguish duplicates caused by high sequencing depth from the PCR artefacts?
One more question. Is it necessary to remove the duplicates in the transcription factor ChIP-Seq? In our TF ChIP-Seqs, It is often to see a high duplication level. It is not the case in the controls. I guess the duplicate is caused by high sequencing depth not by the PCR artefacts.
Wish your help! Thanks very much!
Best, Mei
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