Dear all,
We have experienced a weird issue with our last sequencing run in a Illumina HiScanSQ with a flowcell where samples were multiplexed.
Every lane of the flowcell unless Lane3 have been decoded and converted to FASTQ. We have the bcl files and thumbnail images are present in our system, but surprisingly CASAVA1.8.2 (configureBclToFastq.pl) can't perform the demultiplexing step for that particular lane, so that we can't obtain the correspondent FASTQ files from the two libraries sequenced in that lane.
Has anybody experienced this issue? If you could solve it, would you please give us some advice on how to do it?
We have experienced a weird issue with our last sequencing run in a Illumina HiScanSQ with a flowcell where samples were multiplexed.
Every lane of the flowcell unless Lane3 have been decoded and converted to FASTQ. We have the bcl files and thumbnail images are present in our system, but surprisingly CASAVA1.8.2 (configureBclToFastq.pl) can't perform the demultiplexing step for that particular lane, so that we can't obtain the correspondent FASTQ files from the two libraries sequenced in that lane.
Has anybody experienced this issue? If you could solve it, would you please give us some advice on how to do it?
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