Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Multiple NGS Platform and combining data

    Hi all,

    Would anyone care to comment on the validity of combining data from multiple platforms for a targeted resequencing project. In particular, I am looking at data from 454 and MiSeq for amplicon sequencing project. I am assuming the important things to look for are base calling quality, depth and coverage. If those are high quality then there should be no problem publishing the combined data sets?

    Thanks

  • #2
    Well both machine have different error profiles. So your error filtering protocols will most likely be different. For eg i found that systematic indel errors in 454 amplicon data could severely reduce the total depth of error free data, where MiSeq does not have the same issue. With the MiSeq we get much more depth so low copy number base mismatches, chimeras etc are much easier to distinguish compared to 454 data sets.
    I imagine that after all is said and done and you have run each data set independently through the required QC stages, there is no reason why you can not combine the data

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    59 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    57 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    51 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    56 views
    0 likes
    Last Post seqadmin  
    Working...
    X