Hi All,
We are fairly new to the nextgen seq technology and when we started our bacterial genomes the companies providing sequencing opportunities were unable to do de novo assembly of bacterial genomes with Solexa (shorter read) data. So we choose for 454 at that time.
1) What is the current status on this since? We are planning new sequencing of strains (where we might get away with a resequencing approach) but for one bacterium there is already so much variation between strains...I guess we would need de novo for that.
2) what kind of system requirements would we need (and software) to be able to do this? Can we use a single linux machine? We will not be doing many of these so waiting a day is not a problem.... till now.
Thanks for your vies on this.
We are fairly new to the nextgen seq technology and when we started our bacterial genomes the companies providing sequencing opportunities were unable to do de novo assembly of bacterial genomes with Solexa (shorter read) data. So we choose for 454 at that time.
1) What is the current status on this since? We are planning new sequencing of strains (where we might get away with a resequencing approach) but for one bacterium there is already so much variation between strains...I guess we would need de novo for that.
2) what kind of system requirements would we need (and software) to be able to do this? Can we use a single linux machine? We will not be doing many of these so waiting a day is not a problem.... till now.
Thanks for your vies on this.
Comment