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  • Using DEXSeq with bam files

    Hello everyone,

    I'm working with the programming language R and want to use DEXSeq.
    First I executed tophat on my reads to map them against a human reference genome.
    In R I created a function to load the bam file of tophat:
    Code:
    getCounts <- function( alignmentName, run, tx ){
      fileName <- paste( "/pathToDir/", alignmentName, "/", alignmentName, "run", run, ".merged.bam", sep="" ) #my directory structure
      alignment <- readBamGappedAlignments( fileName )
      newReadNames <- gsub( "([0-9(MT|X|Y)])" , "chr\\1" , rname( alignment ) )
      alignment <- GRanges( seqnames = newReadNames, ranges = IRanges( start = start( alignment ),													   end=end(alignment)), 														   strand=strand(alignment))
      alignmentCounts <- suppressWarnings( countOverlaps( tx,alignment ) )
      return( alignmentcounts )
    }
    I call this function for each replicate and this gives me a dataset with raw counts. These raw counts I get with these code:
    Code:
    txdb <- makeTranscriptDbFromUCSC( genome='hg19', tablename='ensGene' )
    tx_by_exon <- transcriptsBy( txdb, 'exon' )
    tx_by_gene <- transcriptsBy( txdb, 'gene')
    pos1_1 <- getCounts( "pos", 1, tx_by_exon)
    pos1_2 <- getCounts( "pos", 2, tx_by_exon)
    neg1_1 <- getCounts( "neg", 1, tx_by_exon)
    neg1_2 <- getCounts( "neg", 2, tx_by_exon)
    Then I create a dataframe with all counts:
    Code:
    allCounts <- data.frame( pos1_1 = pos1_1, pos1_2 = pos1_2, neg1_1 = neg1_1, neg1_2 = neg1_2, )
    And a dataframe to describe the design:
    Code:
    design <- data.frame(
    		row.names = colnames(allCounts),
    		condition = c( "pos", "pos", "neg", "neg" ),
    		libType = c( "paired-end", "paired-end", "paired-end", "paired-end"))
    And now I stuck...
    To create a exonCountset, I want to use this function:
    Code:
    data <- newExonCountSet(allCounts, design, ?,rownames(allCounts))
    My question is, how do I get the gene IDs of these counts?
    And another question I have is whether it is possible to use the tx_name instead of the tx_id.

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