Hi! everyone! Now i have 2 sRNA high-throughput seqencing library that include 1000,0000 clean reads, i want to search potential siRNA according to criterion that the two perfectly complementary sRNAs with 2 nt overhangs at the 3-end were considered to be siRNA. Tags from clean reads were aligned with each other by blast+,but blast is very slowly! Can anyone tell me some better pipeline or scripts such as perl ? i would be be deeply grateful!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM