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I got too few significantly differently expressed genes between two samples, was something wrong. and how did cuffdiff call a gene significantly differently expressed
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1) How do you know its too few?
2) "two samples" do you mean multiple biological replicates of two conditions or only two samples? It makes a big difference. -
1）I got just 68 genes significantly differently expressed
2) one is cancer cell line,the other is normal tissueComment
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1）I got just 68 genes significantly differently expressedOriginally posted by chadn737 View Post
2) one is cancer cell line,the other is normal tissueComment
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1) Why is 68 too few?
2) So you have only two samples, no replicates?Comment
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