Hi, there:
I got my output from TophatFusion and try to get an idea about how reliable a fusion event is.
From the explanation in the output, there are 3 numbers may contribute to this:
8. Number of spanning reads
9. Number of spanning mate pairs
10. Number of spanning mate pairs where one end spans a fusion
I used IGV to view the bam output from Tophatfusion and confirmed that for 8th, the number means the number of reads which the fusion break point is on the end (which generally called split-fusion read). For 9th, the number means the number of pairs which the fusion break point is between two ends. However, I can't find any information about the 10th number.
Are there anyone has any ideas about this? Or how do you determine how reliable a fusion event is?
Thank you so much
Yuxiang
I got my output from TophatFusion and try to get an idea about how reliable a fusion event is.
From the explanation in the output, there are 3 numbers may contribute to this:
8. Number of spanning reads
9. Number of spanning mate pairs
10. Number of spanning mate pairs where one end spans a fusion
I used IGV to view the bam output from Tophatfusion and confirmed that for 8th, the number means the number of reads which the fusion break point is on the end (which generally called split-fusion read). For 9th, the number means the number of pairs which the fusion break point is between two ends. However, I can't find any information about the 10th number.
Are there anyone has any ideas about this? Or how do you determine how reliable a fusion event is?
Thank you so much
Yuxiang