Hi everyone,
I have datas from a patient sequenced two years ago and now I have datas from the SAME patient re-sequenced last month with a different technology.
Both are exomes.
Maybe some people had the same case, but if I want to do a combine analysis (SNP calling), when do I have to combine the two files ?
1/ concatenate the FASTQ files and then proceed to analysis of the unique file
OR
2/ combine each BAM files (cleaned or not) for examples or any further files?
If somebody had already done this kind of analysis, or maybe any publication with such examples...It will help me a lot.
Thanks very much to the NGS community.
I have datas from a patient sequenced two years ago and now I have datas from the SAME patient re-sequenced last month with a different technology.
Both are exomes.
Maybe some people had the same case, but if I want to do a combine analysis (SNP calling), when do I have to combine the two files ?
1/ concatenate the FASTQ files and then proceed to analysis of the unique file
OR
2/ combine each BAM files (cleaned or not) for examples or any further files?
If somebody had already done this kind of analysis, or maybe any publication with such examples...It will help me a lot.
Thanks very much to the NGS community.
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