Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • BioPerl Issues

    I am having issues with a BioPerl script. I have a blastxml file from a blastx blast and the original multifasta file containing the original nucleotides sequences.

    I want to take the blast result (ie. the blast description) and annotated my multifasta file.

    I have written 2 while loops that extract the blast descriptions as well as the nucleotide sequence from the multifasta file.

    My problem is that I cannot incorporate one of the while loops into the other without loosing the loop property of one of the loops.

    See script below:


    use warnings;
    use strict;
    use Bio::SearchIO;
    use Bio::SeqIO;


    my $search_in = Bio::SearchIO->new(-format => 'blastxml', -file => "$ARGV[0]");
    while (my $result = $search_in->next_result) {
    while (my $hit = $result->next_hit) {
    while (my $hsp = $hit->next_hsp) {
    my $qd = $hit->description;
    print $qd, "\n";
    }
    }
    }

    my $seqio = Bio::SeqIO->new(-format => 'fasta', -file => "$ARGV[1]");
    while (my $seqobj = $seqio->next_seq) {
    my $nuc = $seqobj->seq();
    print $nuc, "\n";
    }

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-10-2024, 06:35 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-09-2024, 02:46 PM
0 responses
21 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-06-2024, 07:17 AM
0 responses
21 views
0 likes
Last Post seqadmin  
Working...
X