Estimate Dispersions with DESeq2
Hello! =)
I'm a beginner (Blutiger Anfänger) at bioinformatics and so I've got a beginners question, sorry!
Nowadays I'm trying to run DESeq2 with a dataset without replicates. It's a dataset with two columns (2 conditions, "control" and "infected").
Which method do you recommend me to estimate the dispersion of my data? ( "pooled", "pooled-CR", "per-condition", "blind"?). I was reading about these methods but it's difficult for me to say which one I should use.![Confused](https://www.seqanswers.com/core/images/smilies/confused.png)
If I just use the command "estimateDispersions" without specifying the method to make use of, which one is applied?
What should I be careful with?
Thanks in advance!
Hello! =)
I'm a beginner (Blutiger Anfänger) at bioinformatics and so I've got a beginners question, sorry!
![Embarrassment](https://www.seqanswers.com/core/images/smilies/redface.png)
Which method do you recommend me to estimate the dispersion of my data? ( "pooled", "pooled-CR", "per-condition", "blind"?). I was reading about these methods but it's difficult for me to say which one I should use.
![Confused](https://www.seqanswers.com/core/images/smilies/confused.png)
If I just use the command "estimateDispersions" without specifying the method to make use of, which one is applied?
What should I be careful with?
Thanks in advance!
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