Hi all!
I'm a newbie in NGS, trying to write a thesis on BRCA mutations on breast cancer, not a biologist, so please excuse my ignorance!
I got one .bam file, of a sample sequenced by Miseq of Illumina and even after indexing it using samtools I see no alignment in IGV. When I used tview in samtools, a string of N's appeared and no bases, nowhere.
Do I need something more than this bam file?
Thank u!
I'm a newbie in NGS, trying to write a thesis on BRCA mutations on breast cancer, not a biologist, so please excuse my ignorance!
I got one .bam file, of a sample sequenced by Miseq of Illumina and even after indexing it using samtools I see no alignment in IGV. When I used tview in samtools, a string of N's appeared and no bases, nowhere.
Do I need something more than this bam file?
Thank u!
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