Hello, I am very confused, I have RNA-seq data and I have mapped it to my assembled genome (still a few hundred contigs) so I have got about 20000 mapped contigs. My question is how can I translate that into transcripts? I mean how can I know two or more contigs belong to the same transcript. Is it possible to know how many genes do I have? Can I use that information to annotate my genome? I have annotated it with predictions and I was expecting to keep only the predictions that I have evidence for in my RNA-seq data but I don't know how to do that. How to make that link? Any help will be very much appreciated, thanks.
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
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