Hi,
1) I'm total a novice in this space.
2) I'm trying to download/read the contents of the data cited here
http://www.ncbi.nlm.nih.gov/geo/download/?acc=GSE34740 specifically GSE34740_K562_align_b_1_GSM854404.bam
3) I created an account so I can use Galaxy
4) I uploaded the BAM file
5) I ran Cufflinks hoping to extract a list of gene names and FPKM values
6) Only the data headers were output. It looks like some kind of problem with the file format?
7) I ran the auto-detect for file format but that didn't changes things.
8) Any ideas?
1) I'm total a novice in this space.
2) I'm trying to download/read the contents of the data cited here
http://www.ncbi.nlm.nih.gov/geo/download/?acc=GSE34740 specifically GSE34740_K562_align_b_1_GSM854404.bam
3) I created an account so I can use Galaxy
4) I uploaded the BAM file
5) I ran Cufflinks hoping to extract a list of gene names and FPKM values
6) Only the data headers were output. It looks like some kind of problem with the file format?
7) I ran the auto-detect for file format but that didn't changes things.
8) Any ideas?