Originally posted by Kath
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I've just got exactly the same with my data. What kit did you use for capture?
Cheers
KathLeave a comment:
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this may help http://www.bioinformatics.babraham.a.../fastq_screen/Leave a comment:
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i don't know why the image is not being shown in the post... here's the link
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Contamination in my sample?
Hi all!
I just ran a 100bp PE human exome seq run in my lab and was looking at the fastqc results of my run. I am getting this weird pattern in the gc content distribution and was wondering is this normal for an exome run or do i have some sort of contamination? I've run an exome seq before in my lab but saw a distribution which was similar to the theoretical distribution with a shorter peak (yet, still normally distributed). The 2 peaks i'm seeing in the new run is really messing with my head as i have 24 samples all showing the same pattern!
Thanks in advance!
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