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  • Cuffmerge very strange errror pattern

    I have a strange error pattern that I'm hoping someone here can shed some light on. I keep getting the following error when running cuffmerge:

    Error: duplicate GFF ID 'M41013' encountered!
    [FAILED]
    Error: could not execute gtf_to_sam
    Traceback (most recent call last):
    File "/cgr/home/qh5653/cufflinks-2.0.2.Linux_x86_64/cuffmerge", line 576, in ?
    sys.exit(main())
    File "/cgr/home/qh5653/cufflinks-2.0.2.Linux_x86_64/cuffmerge", line 554, in main
    sam_input_files = convert_gtf_to_sam(gtf_input_files)
    File "/cgr/home/qh5653/cufflinks-2.0.2.Linux_x86_64/cuffmerge", line 287, in convert_gtf_to_sam
    sam_out = gtf_to_sam(line)
    File "/cgr/home/qh5653/cufflinks-2.0.2.Linux_x86_64/cuffmerge", line 247, in gtf_to_sam
    exit(1)
    TypeError: 'str' object is not callable
    The strange part is, this only occurs under specific circumstances. I have two groups of transcripts.gtf files which I want to merge:

    1) Generated by tophat/cufflinks using publically available reference transcripts - cuffmerge always succeeds

    2) Generated by tophat/cufflinks using custom reference transcripts - cuffmerge always fails

    When I look at the transcripts.gtf files generated by both these pathways, they appear identical (besides the names obviously). Yet one crashes cuffmerge and the other doesn't.

    How is this possible?

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