seeing this message:
[infer_isize] fail to infer insert size: too few good pairs
vast majority of reads/pairs are marked unaligned.
Quality data from SolexaQA doesnt indicate any problems with quality scores. (it also autodetects the scores as standard sanger scores)
These files previously had mapped fairly well to the same reference genome. (in march 2012, when we first received them)
OS: CentOS linux 64 bit nodes on a cluster managed by CCAM group @ UConn Health Center.
i'm currently trying a bowtie2 run to see if I get the same problem. Is there a problem with new versions of BWA? I have references that were built after the 6.0 upgrades.)
I have absolutely no idea whats wrong.
[infer_isize] fail to infer insert size: too few good pairs
vast majority of reads/pairs are marked unaligned.
Quality data from SolexaQA doesnt indicate any problems with quality scores. (it also autodetects the scores as standard sanger scores)
These files previously had mapped fairly well to the same reference genome. (in march 2012, when we first received them)
OS: CentOS linux 64 bit nodes on a cluster managed by CCAM group @ UConn Health Center.
i'm currently trying a bowtie2 run to see if I get the same problem. Is there a problem with new versions of BWA? I have references that were built after the 6.0 upgrades.)
I have absolutely no idea whats wrong.
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