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  • MIRA hybrid assembly error

    Hii all,

    I am trying to assemble a dataset using 454 and illumina PE data. The software aborts the process. I have attached the last of log file obtained.

    Please, help me to sort out this error.

    Thanks


    ""
    makeIntelligentConsensus() complete calc .. mict_fin 2
    mict_pre 63
    mict_shadow 616974
    mict_fallout 12838014
    mict_newin 686089
    mict_helper1 96976912
    mict_restofloop 253445
    mict_totalloop 110990569
    done.



    A file named 'addReaderror_REPLAY_do_not_delete.log' has been written
    to the working directory. Please send it to the author to get that bug fixed!



    Internal logic/programming/debugging error (*sigh* this should not have happened).
    Please file a bug report on http://sourceforge.net/apps/trac/mira-assembler/

    "WARNING/ERROR: contig did not put refid into affected reads. Please file a bug report to
    Download MIRA for free. MIRA - Sequence assembler and sequence mapping for whole genome shotgun and EST / RNASeq sequencing data. Can use Sanger, 454, Illumina and IonTorrent data.

    and please send a mail to
    [email protected]
    "

    ->Thrown: void Contig::addRead(vector<Align> & aligncache, const AlignedDualSeqFacts * initialadsf, int32 refid, int32 newid, int32 direction_frnid, errorstatus_t & errstat)
    ->Caught: void Contig::addRead(vector<Align> & aligncache, const AlignedDualSeqFacts * initialadsf, int32 refid, int32 newid, int32 direction_frnid, errorstatus_t & errstat)

    Aborting process, probably due to an internal error.

    If you want to report the error, please do so on
    Download MIRA for free. MIRA - Sequence assembler and sequence mapping for whole genome shotgun and EST / RNASeq sequencing data. Can use Sanger, 454, Illumina and IonTorrent data.

    and also give a short notice on the mira talk mailing list.

    If reporting, please do not delete the log and checkpoint directories, there may
    be files in them which could be needed to find the problem.

    Subscribing / unsubscribing to mira talk, see: http://www.freelists.org/list/mira_talk

    CWD: /bigdata/krishna/mira/bin
    Thank you for noticing that this is *NOT* a crash, but a
    controlled program stop.
    ""

  • #2
    Given the abort message asks you to contact the MIRA author and gives instructions for how to join the mira_talk mailing list, why are you posting this here instead? Bastein does post here sometimes, but I'm pretty sure this way won't be as quick to reach him.

    You've forgotten to include the important bit of information of which version of MIRA you're using, but this does look like a previously reported bug, which should be fixed in the latest releases (Did you try Google for this?):

    Comment


    • #3
      Thanks for the reply.

      I have mailed the error their too..

      Thanks

      Comment

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