HI everyone. I'm new here. I got a problem blocked me for days and plz help me.
I want to use SOAP to align my paired-end reads. command:
./SOAP -a /home/d-l/L008_R1_001.fastq -b /home/d-l/L008_R2_001.fastq -D /home/d-l/ref.fa.index -o /home/d-l/pairmapped.txt -2 /home/d-l/singlemapped.txt -u /home/d-l/unmapped.txt -m 500 -x 10000
But pairmapped.txt & singlemapped.txt are all size 0. unmapped.txt is large. No read mapped! Then I tried single read:
./SOAP -a /home/d-l/L008_R1_001.fastq -D /home/d-l/ref.fa.index -o /home/d-l/singletest.txt
But still singletest.txt is size 0.
I am going to be crazy. I can't explain to my supervisor. Any one who have succeed?
I want to use SOAP to align my paired-end reads. command:
./SOAP -a /home/d-l/L008_R1_001.fastq -b /home/d-l/L008_R2_001.fastq -D /home/d-l/ref.fa.index -o /home/d-l/pairmapped.txt -2 /home/d-l/singlemapped.txt -u /home/d-l/unmapped.txt -m 500 -x 10000
But pairmapped.txt & singlemapped.txt are all size 0. unmapped.txt is large. No read mapped! Then I tried single read:
./SOAP -a /home/d-l/L008_R1_001.fastq -D /home/d-l/ref.fa.index -o /home/d-l/singletest.txt
But still singletest.txt is size 0.
I am going to be crazy. I can't explain to my supervisor. Any one who have succeed?
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