Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • BFAST, Could not read in buffer

    I'm having some problem getting started with Bfast:
    bob@homequad:/home2/NGS/tmp$ bfast index -n 8 -f /home2/NGS/ref/yeast/seq/S288C_reference_sequence_R64-1-1_20110203.fasta -m 111111111111111111 -w 18 -i 1
    ************************************************************
    Checking input parameters supplied by the user ...
    Validating fastaFileName /home2/NGS/ref/yeast/seq/S288C_reference_sequence_R64-1-1_20110203.fasta.
    Validating tmpDir path ./.
    Input arguments look good!
    ************************************************************
    ************************************************************
    Printing Program Parameters:
    programMode: [ExecuteProgram]
    fastaFileName: /home2/NGS/ref/yeast/seq/S288C_reference_sequence_R64-1-1_20110203.fasta
    space: [NT Space]
    mask: 111111111111111111
    depth: 0
    hashWidth: 18
    indexNumber: 1
    repeatMasker: [Not Using]
    startContig: 0
    startPos: 0
    endContig: 2147483647
    endPos: 2147483647
    exonsFileName: [Not Using]
    numThreads: 8
    tmpDir: ./
    timing: [Not Using]
    ************************************************************
    ************************************************************
    Reading in reference genome from /home2/NGS/ref/yeast/seq/S288C_reference_sequence_R64-1-1_20110203.fasta.nt.brg.
    In total read 17 contigs for a total of 12157105 bases
    ************************************************************
    Creating the index...
    ************************************************************
    Warning: startContig was less than zero.
    Defaulting to contig=1 and position=1.
    ************************************************************
    ************************************************************
    Warning: endContig was greater than the number of contigs in the reference genome.
    Defaulting to reference genome's end contig=17 and position=85779.
    ************************************************************
    Currently on [contig,pos]:
    [------17,------85779]
    Sorting by thread...
    24.998 percent complete************************************************************
    In function "RGIndexMergeHelperFromDiskGetNext_8": Fatal Error[ReadFileError]. Message: Could not read in buffer.
    The file stream error was:: Bad file descriptor
    ***** Exiting due to errors *****
    ************************************************************
    bob@homequad:/home2/NGS/tmp$

    I've looked at the source and can't see right away what the problem
    might be. May I ask for Nils' help?
    Robert Williams

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    Today, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 07:17 AM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
20 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-29-2024, 10:49 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Working...
X