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  • MAQ output format

    Hi users,
    I have some questions about MAQ output file (.snp, generated from cns file), like this:

    hsa-mir-1308 23 C T 27 1 0.00 27 24
    N 3 N
    hsa-mir-1975 31 A R 107 14 1.00 63 62
    A 114 G
    hsa-mir-1975 62 G R 27 3 1.00 63 30
    G 35 A
    hsa-mir-1977 57 T Y 18 11 1.00 63 12
    T 255 C
    hsa-mir-21 38 T G 17 1 0.00 27 4
    N 13 N
    hsa-mir-23b 90 A G 15 2 0.00 27 0
    A 12 R
    hsa-mir-26a-2 36 G C 19 2 0.00 27 16
    G 8 S
    hsa-mir-26a-2 37 T G 17 2 0.00 27 18
    T 6 K
    hsa-mir-26a-2 45 T C 33 2 0.00 27 0
    N 15 N
    hsa-mir-30c-1 50 A G 4 1 0.00 4 0
    N 26 N

    - What is the "read depth" in 6th column?

    - How can I know how many times MAQ counts the mutations (in other words, how many
    times a base call occurs in a specific position)?

    - To find INDELs, (and put them in the output), is there any specific commands?

    - What are the 8th and 9th columns? Are quality parameters?

    Thank you very much,
    Maria Elena

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