Hello all,
I found something potentially interesting in my data where out of 1981 regulated genes (relCDS), just 1 was differentially expressed.
What I want to know is:
How does Cufflinks figure out what CDS's are regulated?
And, is there a way to discover the identity of the regulator?
This is what I found so far, but it doesn't answer my question:
"9.1 Creating gene sets from significantly regulated genes
One of the primary roles of a differential expression analysis is to conduct significance tests on each feature (genes, isoforms, TSS, and CDS) for appropriate pairwise comparisons of conditions. The results of these tests (after multiple testing correction of course) can be used to determine which genes are differentially regulated."
-http://compbio.mit.edu/cummeRbund/manual.html
God bless,
Jason
I found something potentially interesting in my data where out of 1981 regulated genes (relCDS), just 1 was differentially expressed.
What I want to know is:
How does Cufflinks figure out what CDS's are regulated?
And, is there a way to discover the identity of the regulator?
This is what I found so far, but it doesn't answer my question:
"9.1 Creating gene sets from significantly regulated genes
One of the primary roles of a differential expression analysis is to conduct significance tests on each feature (genes, isoforms, TSS, and CDS) for appropriate pairwise comparisons of conditions. The results of these tests (after multiple testing correction of course) can be used to determine which genes are differentially regulated."
-http://compbio.mit.edu/cummeRbund/manual.html
God bless,
Jason
Comment