Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • jeales
    Member
    • Oct 2012
    • 13

    #1

    cummeRbund diffData() or fpkm() values

    I'm getting different FPKM values from diffData() and fpkm() on a couple of different isoform IDs

    Code:
    > diffData(getFeatures(cuff,"TCONS_00246566"))
          isoform_id   sample_1 sample_2  status value_1 value_2 log2_fold_change test_stat p_value q_value significant
    1 TCONS_00246566 macrophage monocyte LOWDATA       0       0    -1.79769e+308         0       1       1          no
    diffData() reports an fpkm of 0 for sample_2 (reported as "value_2")

    Code:
    > fpkm(getFeatures(cuff,"TCONS_00246566"))
          isoform_id sample_name      fpkm conf_hi conf_lo quant_status    stdev
    1 TCONS_00246566  macrophage 0.0000000  0.0000       0      LOWDATA  0.00000
    2 TCONS_00246566    monocyte 0.0972739 49.0851       0      LOWDATA 24.49391
    but fpkm() reports an fpkm of ~0.09 for sample_2

    I know that csHeatmap() uses fpkm() to extract values for plotting, but which is the most accurate value?

    I thought cuffdiff values (available from diffData()) were supposed to be the most accurate?

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...