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  • mra
    Junior Member
    • Feb 2012
    • 7

    Cuffmerge: merging reference and transcript GTFs?

    Dear All.

    After running cufflinks

    Code:
    cufflinks -p 10 --frag-len-mean 400 --frag-len-std-dev 100 -N --GTF-guide ${gtf} --frag-bias-correct ${fasta} --multi-read-correct -o cufflinks_all all.accepted_hits.bam
    I got a 12M transcripts.gtf file that I eventually merged with a 240M reference GTF file using cuffmerge:

    Code:
    cuffmerge -p 10 -o cuffmerge_all --ref-gtf ${gtf} --ref-sequence ${fasta} assemblies.txt
    (on assemblies.txt there is only the transcripts.gtf produced previously by cufflinks)

    Yet, cuffmerge outputs a 142M merged.gtf. Assuming that cuffmerge makes a non-redudant union of the transcripts and reference GTF files, shouldn't merged.gtf weight at least as the reference GTF (240M)?

    Thanks in advance

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