Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • How to identify different bacterial strains in the same sample

    I have Illumina sequencing of a bacterial sample collected from a patient. I suspect it's a mixture of multiple strains of the same bacteria, but I don't which strains are in there. Is there anyway to identify

    1. if this sequence sample is a mixture of different strains?

    2. how many and what strain are in there?

    Thanks.

  • #2
    How to do this in practice: http://seqanswers.com/forums/showthread.php?t=16812

    Analysis (see post #2) in this thread: http://seqanswers.com/forums/showthread.php?t=31565
    Last edited by GenoMax; 07-02-2013, 11:43 AM.

    Comment


    • #3
      Thanks. I'm not sure how this would help in my case. I'm not trying to do any phylogeny analysis. I just need to know if sequence I have was from one pure bacterial strain or from multiple strains.

      Originally posted by GenoMax View Post
      How to do this in practice: http://seqanswers.com/forums/showthread.php?t=16812

      Analysis (see post #2) in this thread: http://seqanswers.com/forums/showthread.php?t=31565

      Comment


      • #4
        Originally posted by shiningway View Post
        Thanks. I'm not sure how this would help in my case. I'm not trying to do any phylogeny analysis. I just need to know if sequence I have was from one pure bacterial strain or from multiple strains.
        My fault. The title on your original post made it sound (to me) like you were interested in identifying bacterial strains not specifically strains of the same species.

        If the strains are expected to have small differences then this would be a difficult problem.
        Last edited by GenoMax; 07-02-2013, 12:43 PM.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Recent Advances in Sequencing Analysis Tools
          by seqadmin


          The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
          05-06-2024, 07:48 AM
        • seqadmin
          Essential Discoveries and Tools in Epitranscriptomics
          by seqadmin




          The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
          04-22-2024, 07:01 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, Today, 07:03 AM
        0 responses
        10 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-10-2024, 06:35 AM
        0 responses
        29 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-09-2024, 02:46 PM
        0 responses
        38 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 05-07-2024, 06:57 AM
        0 responses
        31 views
        0 likes
        Last Post seqadmin  
        Working...
        X