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  • Good poly-A filtering rules or tools

    I am aligning a large number of ESTs. It seems poly-A tails show in many different ways. In addition to occurring at the very end, they can be flanked by the cloning sequence one one end, or have mismatches/errors. What is a good rule or available tools that will handle the usual cases?

    A few examples of the non-trivial cases I found, with their Genbank Accs:

    Code:
    >EE409337
    ... AAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTTGTC
    >EE409340
    ... TTTCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTGTC
    >EE409361
    ... TTGTTAAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAACCATGTCGGC
    TTACTGAATTGAA
    >EE420306
    .... AAAAAAAGTTATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGAAAAAAA
    AAAAAAAAAAAAAAAAA
    Crossposted: StackExchange, Biostars.
    Last edited by ysnapus; 07-05-2013, 03:03 PM. Reason: cross posting

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