Hi,
I am new to NGS data analysis. I have got NGS read data from the Demo run. The Data is from HLA typing using NGS Technology. On looking the read data obtained, the Read 1 and read 2 data where completely complementary to each other. Is it normal ?? I have read that the Paired reads produced by NGS usually overlaps in the 3' region. But can the whole read 1 and read 2 be complementary to each other?
The data was generated using Illumina sequencer. I had read 1 and read 2 in separate files. The read lengths was around 150 bp each. I am not aware of the illumina sequencer details.
I am new to NGS data analysis. I have got NGS read data from the Demo run. The Data is from HLA typing using NGS Technology. On looking the read data obtained, the Read 1 and read 2 data where completely complementary to each other. Is it normal ?? I have read that the Paired reads produced by NGS usually overlaps in the 3' region. But can the whole read 1 and read 2 be complementary to each other?
The data was generated using Illumina sequencer. I had read 1 and read 2 in separate files. The read lengths was around 150 bp each. I am not aware of the illumina sequencer details.
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