The BLAST binaries contain the formatdb utility which is all you need to generate the indices prior to running BLAST
formatdb -p F -d hg18.fa
Would index the nucleotide file (-p F specifies that this is (F)alse for (p)rotein) named hg81.fa
-
Database of BLAST
Hello, everyone.
Now I am doing alignment using BLAST. I want to align some sequences (FASTA format) against human chromosome. I used HG18 from UCSC (<http://hgdownload.cse.ucsc.edu/goldenPath/hg18/chromosomes/>) before with Bowtie. Are there pre-processed db on the FTP of BLAST (ftp://ftp.ncbi.nlm.nih.gov/blast/db/), which is built from HG18? I am a freshman to Bio...
Thanks a lot.
-
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM
Leave a comment: