Hi,
I am wondering how to go about including splice junction's database when generating a genome with STAR. I am using bovine genome downloaded from UCSC (BosTau7/Btau_4.6.1)
I have successfully generated this genome as it is and run mapping jobs against it. I'm just wondering if anyone can point me in the right direction for finding a .gtf file with annotated introns in the three column format:
Chr Start End Strand +/-
I can't see one anywhere on the UCSC site, do I have to create one myself?
Any help would be much appreciated, I'm new to this!
I am wondering how to go about including splice junction's database when generating a genome with STAR. I am using bovine genome downloaded from UCSC (BosTau7/Btau_4.6.1)
I have successfully generated this genome as it is and run mapping jobs against it. I'm just wondering if anyone can point me in the right direction for finding a .gtf file with annotated introns in the three column format:
Chr Start End Strand +/-
I can't see one anywhere on the UCSC site, do I have to create one myself?
Any help would be much appreciated, I'm new to this!
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