Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Simon Anders
    replied
    Well, as long as you have both platforms in both conditions, this is fine. Just treat the library type as a covariate, as in the example in the vignette. (There, we used on purpose a dataset with such a mixture of single-end and paired-end data to demonstrate how to use covariates.)

    Leave a comment:


  • ericfit
    replied
    Thank you for reply.
    I have 10 samples in treatment and 12 samples in untreatment group. In treatment, I have 5 single and 5 pair-ended samples. In untreatment, I have 8 single and 3 pair-ended samples.
    Sorry, I am not the person who designed the experiment. Have no idea why they put it into such way. But I have to do the data analysis.

    Many thanks.

    Leave a comment:


  • Simon Anders
    replied
    No, paired-end is not better in accuracy. At least, for simple differential expression analysis, it does not make much of a difference.

    As for your question, the answer is: It depends.

    Hence, please always give details on your experimental design when posting a question here,

    Leave a comment:


  • Can I put single and pair-ended RNAseq data together in DESeq analysis

    I have RNA-seq data from Illumina GA (single-ended)and Hiseq (pair-ended). Just wonder if I could put them together in differential gene expression analysis. I know pair-end is much better than single-end in accuracy. But not sure if it is reasonable to put them together in the analysis. many thanks

Latest Articles

Collapse

  • seqadmin
    An Introduction to the Technologies Transforming Precision Medicine
    by seqadmin


    In recent years, precision medicine has become a major focus for researchers and healthcare professionals. This approach offers personalized treatment and wellness plans by utilizing insights from each person's unique biology and lifestyle to deliver more effective care. Its advancement relies on innovative technologies that enable a deeper understanding of individual variability. In a joint documentary with our colleagues at Biocompare, we examined the foundational principles of precision...
    01-27-2025, 07:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 10:34 AM
0 responses
15 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-03-2025, 09:07 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 01-31-2025, 08:31 AM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 01-24-2025, 07:35 AM
0 responses
81 views
0 likes
Last Post seqadmin  
Working...
X