If you build your ebwt in the /examples/reference directory
Code:
bowtie2-build lambda_virus.fa lambda_virus
Code:
bowtie2 -x lambda_virus -U ../reads/reads_1.fq -S eg1.sam
10000 reads; of these:
10000 (100.00%) were unpaired; of these:
596 (5.96%) aligned 0 times
9404 (94.04%) aligned exactly 1 time
0 (0.00%) aligned >1 times
94.04% overall alignment rate
10000 (100.00%) were unpaired; of these:
596 (5.96%) aligned 0 times
9404 (94.04%) aligned exactly 1 time
0 (0.00%) aligned >1 times
94.04% overall alignment rate
If you built with bowtie-build instead of bowtie2-build, it /might/ be the problem.
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