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  • BAMBI inference of transcription factor binding sites

    Good morning SeqAnswers Community,

    I am trying to find binding site motifs for a transcriptional regulator. I have used MEME, and that gives me some potentials, but I wanted to try other software to see if I would get other results. BAMBI promises to be the best, of course, by using a Monte Carlo motif-identification algorithm. It has to options for running (C++ version or a MATLAB version). I used the MATLAB version and struggled for a while with trying to get the formatting right (fasta). The key to that was opening up text file up in notepad and deleting all of the spaces between my 10 sequences. However, I am now getting an error,

    "In an assignment A(I) = B, the number of elements in B and
    I must be the same.

    Error in BAMBI (line 363)
    MLpos(c)=b;"

    I prepared a set of random genes in fasta format that gets the same error in case anyone wants to give it a try and see if they get any better.

    Thanks and God bless,
    Jason
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